Table 2

Effect of gemcitabine on formation of cisplatin ICL in the DHFR gene of ovarian cancer cell lines

Cell line	[CDDP]	ICL/10 kb
		−dFdC	+dFdC
	μM
2008	30	0.08	0.12
	60	0.17	0.25
	120	0.39	0.52^2-150
A2780	30	0.05	0.08
	60	0.13	0.18
	120	0.28	0.38^2-150
CP70	100	0.07	0.09
	200	0.14	0.19
	400	0.26	0.37^2-150

Data are from at least two blots, each of three independent biological experiments. Standard errors were in the range of 0 to 0.07 ICL per fragment.

dFdC, gemcitabine; CDDP, cisplatin.
↵2-150 P < 0.05. Significance was evaluated at the highest dose levels.