Increased gene expression changes in Rat-1 fibroblasts, common to all three α1-AR subtypes
| Subtype | ||||
|---|---|---|---|---|
| Accession | α1A-AR | α1B-AR | α1D-AR | |
| Cytokines/growth factors | ||||
| IL-6 | M26744 | 57.5 ± 4.5 | 62 ± 12 | 21.2 ± 2.9 |
| VEGF3 | L20913 | 8.6 ± 3.6 | 16 ± 9.1 | 6.6 ± 2.8 |
| BDNF | D10938 | 6.1 ± 2.3 | 7.3 ± 2.9 | 5.5 ± 1.8 |
| Glioma-derived VEGF | M32167 | 4.4 ± 0.7 | 11.3 ± 3.2 | 3.6 ± 0.1 |
| KGF | X56551 | 4.1 ± 0.1 | 2.1 ± 0.2 | 2.7 ± 0.1 |
| LIF | AB010275 | 2.9 ± 0.3 | 2.1 ± 0.1 | 2.4 ± 0.15 |
| Transcription factors | ||||
| c-fos | X06769 | 63.8 ± 23.4 | 43.5 ± 12.5 | 61 ± 13 |
| c-jun | X17163 | 19.1 ± 0.1 | 23.6 ± 0.1 | 23 ± 1.0 |
| CREM | S66024 | 16.3 ± 0.3 | 10.8 ± 0.5 | 17 ± 0.7 |
| CELF | M65149 | 9.1 ± 0.5 | 17.4 ± 0.3 | 16.9 ± 1.5 |
| NGFI-B | U17254 | 6.4 ± 0.1 | 2.7 ± 0.1 | 6.2 ± 0.1 |
| rNFIL-6 | S77528 | 6.6 ± 0.1 | 5.3 ± 0.4 | 12.4 ± 0.3 |
| Arc | U19866 | 6.6 ± 0.4 | 4.3 ± 0.1 | 10.7 ± 0.7 |
| NF-1-X1 | AB012235 | 4.5 ± 1.7 | 4.8 ± 1.8 | 7.9 ± 3.3 |
| MSX-2 | U12514 | 3.9 ± 0.3 | 2.3 ± 0.2 | 2.0 ± 0.2 |
| rHox | S82911 | 2.6 ± 0.1 | 2.3 ± 0.2 | 3.4 ± 0.1 |
| RNR-1 | L08595 | 2.3 ± 0.2 | 4.1 ± 0.7 | 11.0 ± 1.2 |
| NGFI-A (Egr-1, Krox-24) | M18416 | 2.1 ± 0.1 | 3.1 ± 0.2 | 2.7 ± 0.1 |
| Krox-20 (Egr-2) | U78102 | 2.0 ± 0.1 | 2.2 ± 0.1 | 2.8 ± 0.3 |
| Krox-24 3′-UTR (Egr-1, NGFI-A) | U75397 | 2.0 ± 0.1 | 2.7 ± 0.2 | 2.5 ± 0.1 |
| Enzymes | ||||
| Tyrosine phosphatase-CL100 | S74351 | 12.9 ± 1.5 | 13.4 ± 1.4 | 14.4 ± 1.7 |
| Tyrosine phosphatase-BAD2 | U02553 | 8.5 ± 0.2 | 6.7 ± 0.5 | 7.6 ± 0.0 |
| Stearoyl-CoA-desaturase 2 | AF036761 | 6.9 ± 0.5 | 9.4 ± 0.1 | 10.7 ± 0.6 |
| Collagenase | M60616 | 5.9 ± 0.2 | 8.4 ± 0.6 | 3.6 ± 0.4 |
| Hexokinase | D26393 | 4.9 ± 1.7 | 4.4 ± 1.0 | 7.1 ± 1.5 |
| proCathepsin L | S85184 | 2.1 ± 0.1 | 2.7 ± 0.3 | 4.1 ± 0.2 |
| Calcineurin A α | D90035 | 2.0 ± 0.0 | 2.2 ± 0.1 | 2.6 ± 0.1 |
| Extracellular matrix proteins | ||||
| Fibronectin | M28259 | 4.4 ± 1.5 | 3.7 ± 1.1 | 3.8 ± 1.3 |
| Collagen III | M21354 | 2.4 ± 0.2 | 2.1 ± 0.1 | 4.6 ± 0.2 |
Data indicate the fold increase ± S.E.M. in gene expression with the indicated accession number. For each gene cluster, the genes are listed in decreasing order of magnitude, taking into account the α1A-AR fold values as reference point. Data are from two separate hybridizations from two separate RNA preparations and compared in a four-way analysis as described under Materials and Methods. Fold changes are similar among the subtypes.
BDNF, brain-derived neurotrophic factor; KGF, keratinocyte growth factor; CREM, cAMP response element modulator protein; CELF, C/EBP-like factor; NGFI-B, nerve growth factor-induced B factor; rNFIL-6, rat nuclear factor induced by IL-6; MSX, muscle-segment homeobox; RNR, ribonucleotide reductase; NGFI-A, nerve growth factor-induced protein A; UTR, untranslated region.