Activity of functional domains of NOS (reductase and oxygenase) and DT-diaphorase in MDA231 parental and NOSII-transfected clones
| All values are mean ± S.D. of at least triplicate experiments. | |||
|---|---|---|---|
| Clone | NOS Reductase | NOS Oxygenase | DT-Diaphorase |
| nmol of cyt c / min / mg | pmol of citrulline / min / mg | nmol of cyt c / min / mg | |
| Parental | 3.53 ± 0.37 | 0.87 ± 0.11 | 8.86 ± 0.94 |
| Vector control | 4.86 ± 0.70 | 0.78 ± 0.17 | 7.72 ± 0.67 |
| iNOS-9 | 6.71 ± 0.20 | 11.62 ± 0.93 | 8.45 ± 1.11 |
| iNOS-12 | 14.96 ± 0.63 | 32.30 ± 1.58 | 9.86 ± 1.03 |
| iNOS-8 | 21.15 ± 1.41 | 64.51 ± 2.41 | 10.12 ± 2.4 |
| iNOS-10 | 22.11 ± 1.63 | 66.20 ± 3.11 | 6.86 ± 1.41 |