Changes in hydrophobicity and size for the mutated channels
| Hydrophobicity values given as difference between hydropathy indices for substituted residue and wild-type residue (Kyte and Doolittle, 1982). Positive values imply increased hydrophobicity. Size change given as change in residue volume, calculated from densitometric measurements (Zamyatnin, 1972). | |||
|---|---|---|---|
| Channel | Substitutions | Hydrophobicity Change | Size Change |
| S6 Mutations | |||
| Mu395 | G395S | -0.4 | +48 |
| C398A | -0.7 | -18 | |
| Mu404 | V404T | -4.9 | -17 |
| Mu409 | I409V | -0.3 | -16 |
| I411V | -0.3 | -16 | |
| N414S | +4.3 | -24 | |
| Mu417 | S417N | -2.7 | +32 |
| E418Y | +2.2 | +40 | |
| P-loop Mutations | |||
| Mu366 | A366D | -5.3 | +25 |
| S367A | +2.6 | -12 | |
| Mu372 | T372V | +4.9 | +20 |
| I373V | -0.3 | -16 | |
| T374S | -0.1 | -23 | |
| Mu383 | I383M | -2.6 | -2 |
| Y384V | +5.5 | -18 | |