TABLE 3

Affinities (pK_A) of the allosteric agents to bind to the indicated wild-type and mutant receptors and factors of cooperativity (pα) for the interaction with [³H]NMS equilibrium binding

pK_A is the -log equilibrium dissociation constant for allosteric agent binding to NMS-free receptors. pα is the -log value of the cooperativity factor; a positive or negative pα reflects an elevation or reduction of [³H]NMS equilibrium binding, respectively. Data are presented as means ± S.E. of three to four separate experiments with duplicated values.