Effect of AGRP fragments on 125I-NDP-MSH binding at the HEK cells transfected with TM chimera of the hMC4R
| 125I-NDP-MSH | ||||
|---|---|---|---|---|
| max | NDP-MSH (i) | AGRP 87-132 (i) | AGRP 110-117 (i) | |
| hMC4RWT | 325±5.8 | 2.16±0.20 | 8.31±0.31 | 20.0±2.2 |
| hMC4R/TM3 hMC1R | 298±21.5 | 1.74±0.20 | 38.1±3.41 | >103 |
| hMC4R/TM4 hMC1R | 278±16.4 | 4.31±0.11 | 105±8.23 | >103 |
| hMC4R/TM5 hMC1R | 301±11.7 | 1.92±0.32 | 10.3±2.12 | 71.3±7.35 |
| hMC4R/TM6 hMC1R | 295±22.5 | 4.70±0.20 | 14.0±3.40 | 25.7±4.5 |
| hMC1RWT | 354±6.7 | 1.32±0.09 | >103 | No binding |