Inhibition of Hoechst 33342 efflux by various inhibitors
All inhibitor concentrations were 10 μM, and experiments were repeated multiple times (n) as indicated. Inhibitor was able to decrease the percentage of Hoechst 33342, excluding cells in the `E' gate, by ≥ 25% (+), by ≥ 50% (++), by ≥ 75% (+++), or by 100% (++++).
| Inhibitor (n) | Effectiveness of Reversal in ABCG2 Cells |
|---|---|
| Reserpine (10) | ++++ |
| MK571 (12) | ++ |
| LY171883 (6) | + |
| Cyclosporine A1 + cremophor (10) | + |
| Cyclosporine A1 (8) | + |
| Verapamil (2) | +/− |
| PSC833 (3) | +/− |
| Structures related to reserpine | |
| Yohimbine (2) | − |
| Corynantheine (2) | − |
| Leukotrienes | |
| B4 (4) | − |
| C4 (4) | − |
| D4 (4) | − |
| Miscellaneous compounds | |
| BSO (1) | − |
| Progesterone (1) | − |
| Probenecid (1) | − |
| Prostaglandin A1 (1) | − |
−, no change.