TABLE 2

Correlation of baseline mRNA expressions of genes to IC50 values of ART in 55 cell lines of the NCI

Symbol Name Correlation to IC50 of ARTa
P Value R Valueb
Drug Resistance Genes
GLCLR Glutamate-cysteine ligase, regulatory (30.8 kD), γ-glutamylcysteine synthetase 0.00051 0.30452
GSTT2 Glutathione S-transferase T2 0.00136 0.27787
MGST3 Microsomal glutathione S-transferase 3 0.00246 0.27144
TOP1 DNA topoisomerase I 0.00001 −0.38821
=4/63 genes (=6%)
DNA Damage and Repair Genes
ERCC5 Excision repair cross-complementing rodent repair deficiency, complementation group 5 0.00074 −0.29471
UNG Uracil-DNA glycosylase 0.00116 −0.28233
FEN1 Flap structure-specific endonuclease 1 0.00014 −0.33695
LIG1 ATP-dependent DNA ligase I 0.00037 −0.31290
RPS3 Ribosomal protein S3 <0.00001 −0.41150
UBE2A Ubiquitin-conjugating enzyme E2A; RAD6 homolog 0.00236 0.26201
HMG1 High-mobility group 1 0.00049 −0.30555
HMG17 High-mobility group 17 0.00085 0.29403
=8/54 genes (=15%)
Oncogenes and Tumor Suppressor Genes
BRCA2 Breast cancer susceptibility gene 2 0.00094 −0.28832
EGFR Epidermal growth factor receptor 0.00390 0.24678
EMP1 Epithelial membrane protein 1 0.00457 0.24412
FOSL2 FOS-like antigen-2 0.00166 0.27229
IRF4 Interferon regulatory factor 4 0.00156 −0.27402
MEN1 Multiple endocrine neoplasia 1 0.00278 −0.25719
MYB v-myb avian myeloblastosis viral oncogene homolog 0.00006 −0.36127
MYC v-myc avian myelocytomatosis viral oncogene homolog 0.00002 −0.38498
CSK c-src tyrosine kinase 0.00142 −0.27672
TGFB2 Tumor growth factor β2 0.00345 0.25816
FOSB FBJ murine osteosarcoma viral oncogene homolog B 0.00456 0.24187
BRAF v-raf murine sarcoma viral oncogene homolog B1 0.00031 −0.32033
RAB2 Member RAS oncogene family 2 0.00047 0.30686
RAN Member RAS oncogene family 0.00315 −0.25330
ARHC RAS oncogene family member C 0.00004 0.36634
ARHE RAS oncogene family member E 0.00097 0.28755
=16/112 genes (=14%)
Apoptosis-Regulating Genes
LOC51272 Locus 51272 0.00153 0.27457
CIDEB Cell death-inducing DFFA-like effector b 0.00022 −0.32566
PDCD2 Programmed cell death 2 0.00040 −0.31118
BAG1 BCL2-associated athanogene 0.00045 −0.29123
BAG3 BCL2-associated athanogene 3 0.00505 0.23856
MADD MAP-kinase activating death domain 0.00720 −0.22917
=5/65 genes (=8%)
Proliferation-Associated Genes
DNL Dynein, cytoplasmic, light polypeptide 0.00039 0.31182
KIF5B Kinesin family member 5B 0.00005 0.36333
TUBB4 β-Tubulin 4 0.00260 0.15057
LOC57018 Cyclin L ania-6a 0.00558 −0.23537
CDK8 Cyclin-dependent kinase 8 0.00019 −0.32938
CDKN3 Cyclin-dependent kinase inhibitor 3 0.00262 −0.26151
CDC25A Cell division cycle 25A gene 0.00055 −0.30264
CEP3 CDC42 effector protein 3 0.00212 0.26513
SPEC1 Small protein effector 1 of CDC42 0.00065 0.30143
APC5 Anaphase-promoting complex subunit 5 0.00109 −0.28426
APC7 Anaphase-promoting complex subunit 7 0.00200 −0.26684
GSPT1 G1 to S phase transition 1 gene 0.00449 −0.24230
MPHOSPH10 M-phase phosphoprotein 10 0.00259 −0.25931
MPHOSPH6 M-phase phosphoprotein 6 0.00069 −0.29662
PA2G4 Proliferation-associated 2G4 gene, 38 kD 0.00003 −0.36954
CTGF Connective tissue growth factor 0.00138 0.27747
VEGFC Vascular endothelial growth factor C 0.00125 0.28038
=17/123 genes (=14%)
Cytokines
SCYB13 Small inducible cytokine B subfamily, member 13 0.00178 0.27571
IL13RA1 Interleukin 13 receptor α 1 0.00001 0.39040
IL6ST Interleukin 6 signal transducer (gp 130, oncostatin M receptor) 0.00131 0.27905
CSF1 Colony-stimulating factor 1 0.00001 0.39986
=4/48 genes (=8%)
Total: 54/465 genes (= 12%)

  • ↵ a Significance (P values) and correlation coefficients (R values) were calculated by Kendall's τ test

  • ↵ b Negative R values indicate that mRNA expression correlates inversely with IC50 values for ART; positive R values indicate a direct correlation of both parameters.