TABLE 2
Pharmacological profile of InsP3 and cAMP production in CHO
cells expressing the NTS1 receptor
Inhibition constants (Ki) were determined from data
obtained in competition experiments with 125I-NT (50 pM) by using
the Cheng-Prusoff equation, with a Kd value for
125I-NT (obtained in saturation binding experiments) of 0.12 nM.
Maximal binding capacities were 2.9 and 0.65 pmol of 125I-NT
binding sites/mg of protein for CHO-NTR-H and CHO-NTR-L cells, respectively.
Potency (EC50) of each agonist to increase cAMP or InsP3
production was determined from concentration-response curves as described
under Materials and Methods. Values represent the means ±
S.E.M. of three to four independent experiments.