TABLE 3

Effect of classic DAT inhibitors on CHO cells stably expressing WT or D79E DAT

K_d (WIN 35,428 only) and K_i values were derived from experiments incubating the stably-transfected cells with nonradioactive substrates in the presence of [³H]dopamine or [³H]WIN 35,428 at 22° C in KRH buffer. Values are presented as mean ± S.E. for three to six independent experiments. Note that values are nanomolar, whereas those in Table 2 are micromolar.

	K_d or K_i
	WT DAT	D79E DAT
	nM
[³H]DA uptake
WIN 35,428	74 ± 17	71 ± 12
Cocaine	555 ± 29	1126 ± 32^a
Mazindol	31 ± 3	47 ± 4
Methylphenidate	152 ± 20	161 ± 9
[³H]WIN inhibition
WIN 35,428	20 ± 1^b	68 ± 4^a
Cocaine	128 ± 5^b	1066 ± 84^a
Mazindol	13 ± 1^b	41 ± 3^a
Methylphenidate	57 ± 3^b	167 ± 2^a

↵ a P < 0.05 versus WT DAT for that assay (via Student's t test).
↵ b P < 0.05 for WIN inhibition versus dopamine uptake at the same DAT construct (via Student's t test).