Primers used for amplification of EPHX2 exons Sequencing primers are shown in upper cases.
| Exon | Size | Forward Primer Sequences | Reverse Primer Sequences |
|---|---|---|---|
| bp | |||
| 1 | 416 | 5′GTTTTCCCAGTCACGACGcggagtcccgttaagggggtgt-3′ | 5′-AGGAAACAGCTATGACCATtcagggccaggtcttcagtttcat-3′ |
| 2 | 463 | 5′-GTTTTCCCAGTCACGACGgtgaagctgctattaggtggt-3′ | 5′-AGGAAACAGCTATGACCATatggtgggccccaagttcggggagat-3′ |
| 3 | 416 | 5′-GTTTTCCCAGTCACGACGgaattgcttggtcacagggaat-3′ | 5′-AGGAAACAGCTATGACCATtccatcgtggctcagaagatctgt-3′ |
| 4 | 546 | 5′-GTTTTCCCAGTCACGACGacctgcttggcccatcattgg-3′ | 5′-AGGAAACAGCTATGACCATtgtaagagctatacaatggagcca-3′ |
| 5 | 500 | 5′-GTTTTCCCAGTCACGACGctgtgtggggtcatatgtgcctt-3′ | 5′-AGGAAACAGCTATGACCATcgtgctgactgcacagtgggtg-3′ |
| 6 | 400 | 5′-GTTTTCCCAGTCACGACGagaactcagtcttggggctccac-3′ | 5′-AGGAAACAGCTATGACCATctgctggtcgttttaggtgacctg-3′ |
| 7 | 364 | 5′-GTTTTCCCAGTCACGACGcatgcacaatccagcaacggactg-3′ | 5′-AGGAAACAGCTATGACCATgagacaatgctccactgtcaggtt-3′ |
| 8 | 401 | 5′-GTTTTCCCAGTCACGACGggtagtgccctgtggctcttggt-3′ | 5′-AGGAAACAGCTATGACCATcagagacacagggcaccgtag-3′ |
| 9 | 457 | 5′-GTTTTCCCAGTCACGACGcacagatggatatgacatcaaaagc-3′ | 5′-AGGAAACAGCTATGACCATgtcgatgctgcatacatgtgggc-3′ |
| 10 | 370 | 5′-GTTTTCCCAGTCACGACGtgggggttaggacttcaacacag-3′ | 5′-AGGAAACAGCTATGACCATttctccacgcaatccttgctctttgt-3′ |
| 11 | 489 | 5′-GTTTTCCCAGTCACGACGcttggtctcctaagcagatgtaa-3′ | 5′-AGGAAACAGCTATGACCATgtcaccagacactaaagagtgaag-3′ |
| 12 | 636 | 5′-GTTTTCCCAGTCACGACGcactgctagtgtatgaccttgtg-3′ | 5′-AGGAAACAGCTATGACCATcttagaccctggtgccataatc-3′ |
| 13 | 400 | 5′-GTTTTCCCAGTCACGACGggaactacagttagcatcttgta-3′ | 5′-AGGAAACAGCTATGACCATtcctggtccctgccacacacta-3′ |
| 14 | 382 | 5′-GTTTTCCCAGTCACGACGcagtctgcgtggtactcagaggtca-3′ | 5′-AGGAAACAGCTATGACCATgagtcagccctatgcatgccg-3′ |
| 15 | 450 | 5′-GTTTTCCCAGTCACGACGggccttgcgcaagttcagatg-3′ | 5′-AGGAAACAGCTATGACCATctgagctatcccaaactagagcag-3′ |
| 16 | 408 | 5′-GTTTTCCCAGTCACGACGcttgctgctagagcttggcaac-3′ | 5′-AGGAAACAGCTATGACCATagcagatctgtccacaaccagctct-3′ |
| 17 | 329 | 5′-GTTTTCCCAGTCACGACGgatacaacgtcaggaccacagc-3′ | 5′-AGGAAACAGCTATGACCATccacatgacaaggtgtatcttct-3′ |
| 18,19 | 685 | 5′-GTTTTCCCAGTCACGACGgcaagcagcaaccgtgactgta-3′ | 5′-AGGAAACAGCTATGACCATgccatccatgcaagatgtgtgta-3′ |