TABLE 1
Binding properties of WT rmGlu5a and mutants
Saturation binding isotherms of [3H]MPEP were performed on membrane preparations from HEK-293 cells transiently transfected with the WT and point-mutated receptors as described under Materials and Methods. With exception of the mutants P654S and P654S/S657C, which are from four independent experiments, the values for other mutants are mean ± S.D. of the KD and Bmax, calculated from three independent experiments, each performed in triplicate. The mutations that affected the [3H]MPEP binding affinity are in bold. Statistical significance was determined using the two-tailed t test.