Activation | Steady-State Inactivation | ||||
---|---|---|---|---|---|
V0.5 | Slope (s) | V0.5 | Slope (s) | n | |
mV | mV | mV | mV | ||
WT | -32.7 ± 1.0 | 5.4 ± 0.2 | -76.8 ± 0.7 | 5.6 ± 0.1 | 30 |
I-S6 | |||||
I424C | -31.2 ± 1.5 | 4.6 ± 0.4 | -73.1 ± 1.3 | 5.5 ± 0.3 | 14 |
I425C | -26.1 ± 0.7* | 7.6 ± 0.2* | -74.4 ± 0.6 | 5.9 ± 0.3 | 14 |
F426C | -33.6 ± 1.7 | 4.9 ± 0.3 | -82.8 ± 1.2* | 6.1 ± 0.3 | 9 |
L427C | -34.6 ± 1.0 | 5.2 ± 0.3 | -74.0 ± 1.8 | 5.1 ± 0.2 | 11 |
G428C | -32.3 ± 1.0 | 6.1 ± 0.4 | -75.9 ± 0.6 | 5.3 ± 0.1 | 11 |
S429C | -32.4 ± 2.6 | 4.5 ± 0.3 | -74.0 ± 1.1 | 5.8 ± 0.4 | 12 |
F430C | -31.2 ± 1.7 | 5.1 ± 0.4 | -81.2 ± 1.1* | 5.9 ± 0.3 | 6 |
Y431C | -31.8 ± 1.4 | 4.2 ± 0.5 | -70.8 ± 1.1* | 5.7 ± 0.1 | 13 |
L432C | -29.5 ± 1.7 | 6.5 ± 0.4* | -73.0 ± 0.9 | 6.0 ± 0.2 | 11 |
I433C | -30.2 ± 1.2 | 6.3 ± 0.4 | -74.7 ± 1.2 | 5.6 ± 0.2 | 6 |
N434C | -30.2 ± 1.1 | 6.3 ± 0.2 | -82.0 ± 0.7* | 6.1 ± 0.2* | 18 |
L435C | -33.9 ± 1.6 | 4.5 ± 0.3 | -77.8 ± 1.7 | 5.8 ± 0.3 | 10 |
I436C | -24.9 ± 1.2* | 5.5 ± 0.5 | -71.9 ± 0.7* | 5.1 ± 0.3 | 10 |
II-S6 | |||||
V779C | -36.6 ± 1.1* | 4.6 ± 0.2 | -82.5 ± 1.5* | 5.6 ± 0.1 | 15 |
M780C | -29.1 ± 2.0 | 4.2 ± 0.2 | -76.5 ± 1.9 | 6.3 ± 0.2* | 10 |
V781C | -34.3 ± 1.9 | 4.5 ± 0.3 | -74.9 ± 1.8 | 5.5 ± 0.3 | 12 |
I782C | -33.5 ± 2.0 | 4.5 ± 0.6 | -68.6 ± 0.6* | 5.1 ± 0.3 | 8 |
G783C | -34.2 ± 1.4 | 4.1 ± 0.2 | -79.4 ± 2.7 | 6.2 ± 0.2* | 8 |
N784C | -33.8 ± 1.0 | 4.5 ± 0.4 | -74.1 ± 1.1 | 6.0 ± 0.3 | 8 |
L785C | -26.0 ± 1.4* | 4.0 ± 0.2 | -74.3 ± 0.8 | 5.4 ± 0.1 | 14 |
V786C | -35.9 ± 1.6 | 4.1 ± 0.7 | -69.1 ± 1.6* | 4.4 ± 0.2* | 7 |
V787C | -27.3 ± 1.2* | 6.5 ± 0.5* | -76.3 ± 0.6 | 5.5 ± 0.1 | 12 |
L788C | -33.4 ± 1.8 | 4.3 ± 0.3 | -77.4 ± 0.8 | 5.7 ± 0.2 | 7 |
N789C | -28.5 ± 1.1 | 4.2 ± 0.2 | -74.3 ± 0.9 | 5.9 ± 0.2 | 9 |
L790C | -33.1 ± 1.7 | 5.0 ± 0.5 | -72.6 ± 1.0* | 5.3 ± 0.2 | 9 |
F791C | -35.0 ± 0.9 | 5.4 ± 0.5 | -74.1 ± 1.0 | 5.1 ± 0.1 | 7 |
↵* P < 0.05 compared with wild-type (WT).