TABLE 2

Effect of peptides modified from WKYMVm-NH₂ on intracellular calcium increase in FPRL1-expressing RBL-2H3 cells

Intracellular calcium increase was monitored from fura-2-loaded cells as described under Materials and Methods.

Sequence	EC₅₀
	nM
WKYMVm-NH₂	0.60 ± 0.090
WKGMVm-NH₂	21.32 ± 2.104^a
WKYMGm-NH₂	18.11 ± 1.308^a
WKYMVG-NH₂	5,945.8 ± 176.100^a
WRYMVm-NH₂	0.12 ± 0.010^a
WEYMVm-NH₂	5.23 ± 0.196^a
WHYMVm-NH₂	0.72 ± 0.075
WDYMVm-NH₂	14.28 ± 1.225^a
WKHMVm-NH₂	1.94 ± 0.268^a
WKEMVm-NH₂	28.30 ± 1.354^a
WKWMVm-NH₂	0.16 ± 0.027^a
WKRMVm-NH₂	2.06 ± 0.256^a
WKDMVm-NH₂	8.73 ± 1.210^a
WKFMVm-NH₂	0.23 ± 0.042^a
WKYMYm-NH₂	0.29 ± 0.061^a
WKYM(F/W)m-NH₂	0.12 ± 0.015^a
WKYMVE-NH₂	502.87 ± 64.965^a
WKYMVV-NH₂	1,259.15 ± 95.750^a
WKYMVR-NH₂	177.52 ± 26.035^a
WKYMVW-NH₂	194.48 ± 19.210^a
WKYMV-NH₂	917.85 ± 45.610^a
KYMVm-NH₂	3.01 ± 0.232^a
KYMV-NH₂	>30,000
YMVm-NH₂	17.15 ± 0.889^a
MVm-NH₂	>30,000
wkymvm-NH₂	Inactive

↵ a p < 0.05 compared with WKYMVm.