TABLE 1

Whole cell [³H]NMS saturation binding to determine M₃ mAChR expression in plasmid control (P3), ^K220RGRK2, ^K220RGRK3, ^K215RGRK5, and ^K215RGRK6 clones The B_max and K_D values were determined using nonlinear regression analysis and curve-fitting programs of GraphPad Prism 3. The data are expressed as the means of specific binding ± S.E.M. for three to five separate experiments.