TABLE 2

Inhibition of GTN denitration in hepatic mitochondria by cyanamide and propionaldehyde, and in mitochondria from GTN-tolerant rats Samples were incubated with 200 nM GTN in the presence or absence of NAD+ for 0 or 20 min, and the GDN metabolites were quantitated. Values represent the mean ± S.D. (n = 3—6).

1,3-GDN 1,2-GDN
Sample NAD+ 0 min 20 min 0 min 20 min
nM
Control
   Mitochondria - 0.9 ± 0.9 1.7 ± 1.1 5.6 ± 4.4 9.1 ± 9.7
   Mitochondria + 0.7 ± 0.7 1.5 ± 1.0 18.8 ± 7.7* 29.8 ± 14.3*
   Mitochondria + 1 mM cyanamide + 0.3 ± 0.5 1.2 ± 1.6 3.3 ± 2.5 0.9 ± 1.0
   Mitochondria + 1 mM propionaldehyde + 0.3 ± 0.6 0.9 ± 0.8 0.0 ± 0.8 2.5 ± 2.0
Tolerant
   Mitochondria - 1.4 ± 1.7 1.7 ± 2.8 1.1 ± 0.3 3.6 ± 2.4
   Mitochondria + 0.5 ± 0.8 1.1 ± 1.8 1.4 ± 0.7 3.7 ± 2.2
  • * P < 0.05 versus all other groups (one-way analysis of variance). All other comparisons were not significantly different