Effects of SSAT-directed siRNA on DENSPM-induced changes in polyamine metabolism in SK-MEL-28 cells
| Treatment | Enzyme Activity | Polyamine Pools | ||||||
|---|---|---|---|---|---|---|---|---|
| SSAT | ODC | Put | AcSpd | Spd | DASpm | Spm | DENSPM | |
| pmol / min / mg | nmol / h / mg | pmol / 106 cells | ||||||
| Control siRNA | 20 ± 2 | 0.47 ± 0.12 | <10 | <10 | 650 ± 360 | <10 | 5,290 ± 320 | |
| siSSAT200 | 12 ± 5 | 0.54 ± 0.04 | <10 | <10 | 870 ± 265 | <10 | 5,250 ± 210 | |
| Control siRNA + DENSPM | 28,040 ± 3,380 | 0.05 ± 0.01 | 685 ± 225 | 465 ± 40 | 30 ± 15 | 135 ± 45 | 325 ± 80 | 11,766 ± 2908 |
| SiSSAT200 + DENSPM | 2,910 ± 550 | 0.03 ± 0.01 | 230 ± 130 | 330 ± 30 | 1,385 ± 70 | <10 | 2,020 ± 195 | 7308 ± 766 |