Summary of cDNA constructs made as templates for producing BCRP cRNA
| Construct | Plasmid | Features of the BCRP cRNA Produced | RNA Polymerase Consensus Sequence Upstream from BCRP cDNA |
|---|---|---|---|
| I | pSP64poly(A) | R482T-poly(A) | SP6 |
| II | pCR Blunt TOPO II | Kozak-R482T-poly(A) | T7 |
| III | pSD64TR | 5′UTR-Kozak-R482T-3′UTR-poly(A) other BCRP forms: 5′UTR-Kozak-R482-3′UTR-poly(A) 5′UTR-Kozak-R482T/S187A-3′UTR-poly(A) 5′UTR-Kozak-R482T/S187T-3′UTR-poly(A) | SP6 |
| III- | pSD64TR | 5′UTR-R482T-3′UTR-poly(A) | SP6 |
5′UTR, 3′UTR, portions of the 5′- and 3′-UTR of the Xenopus laevis β-globin gene; Kozak, modified sequences proximate to the start codon, as described under Materials and Methods: Poly(A), addition of a poly(A) tail, as described under Materials and Methods.