Relative transcript levels of bcl-XL and NF-ϰB in cells exposed to flavone or camptothecin
Transcript levels are given as the ratios of band intensities (area under the curve) of amplified cDNAs from target genes versus GAPDH (used as a constitutively expressed control). Before semiquantitative RT-PCR, cells were treated with medium alone (control) or with 150 μM flavone or 25 μM camptothecin for the times indicated.
| Time of Treatment | ||||
|---|---|---|---|---|
| Treatment | 3 h | 8 h | 24 h | 48 h |
| bcl-XL | ||||
| Control | 2.10 ± 0.31 | 2.30 ± 0.39 | 2.17 ± 0.34 | 2.25 ± 0.12 |
| Flavone | 2.31 ± 0.20 | 2.41 ± 0.32 | 1.99 ± 0.21 | 2.05 ± 0.33 |
| Camptothecin | 2.13 ± 0.19 | 1.14 ± 0.13* | 0.85 ± 0.06* | 0.80 ± 0.10** |
| NF-ϰB | ||||
| Control | 1.54 ± 0.16 | 1.65 ± 0.22 | 1.86 ± 0.17 | 1.61 ± 0.16 |
| Flavone | 1.56 ± 0.25 | 1.36 ± 0.19 | 1.65 ± 0.15 | 1.84 ± 0.25 |
| Camptothecin | 1.53 ± 0.20 | 1.55 ± 0.23 | 0.92 ± 0.16* | 0.85 ± 0.22* |
*, P < 0.05; **, P < 0.001 when compared with controls (n = 3).