TABLE 1

Effects of YC-1 and rolipram, with and without PGE₁, on the peak [Ca²⁺]_i and the time taken for this concentration to decline to half of its peak value (t_1/2) in FMLP-activated neutrophils

Neutrophils labeled with fluo-3 AM as described under Materials and Methods were stimulated with 0.1 μM FMLP in the presence of 1 mM Ca²⁺, and fluorescence was monitored at 37°C with stirring. All data are expressed as mean ± S.E.M.

Drug	Peak [Ca²⁺]_i	t_1/2
	nM	s
Control	366.00 ± 19.66	30.17 ± 0.87
YC-1 3 μM	348.67 ± 24.33	27.20 ± 1.07^*
YC-1 10 μM	359.00 ± 19.29	19.60 ± 0.99^**
Rolipram 1 μM	348.00 ± 21.36	26.40 ± 0.31^*
PGE₁ 0.03 μM	362.00 ± 20.13	29.80 ± 0.64
PGE₁ 0.1 μM	352.00 ± 25.89	22.67 ± 1.54^*
YC-1 3 μM + PGE₁ 0.03 μM	361.00 ± 13.43	18.77 ± 1.13^**
Rolipram 1 μM + PGE₁ 0.03 μM	362.33 ± 25.64	18.23 ± 0.96^**

^*P < 0.05, and ^**P < 0.01 compared with the control.