Comparison of the different CYP2B1 promoter sequences used in heterologous SV40-promoter-luciferase constructs The NR1 element is bold; mutations are lower case. For sticky-end ligation into the pGL3-SV40-promoter vector (Acc65I/BglII sites), double-stranded oligonucleotides with additional four-base, sticky-end overhangs were used.
| Plasmid | Distal CYP2B1 Promoter Sequence |
|---|---|
| pGL3CS2 | 5′-GCACAGACTCTGTACTTTCCTGACCTTG- |
| GCACAGTGCCACCATCAACTTGACTGACACCAG-3′ | |
| pGL3CM4 | 5′-GCACAGACTCTGTACTTTCCTGACCTTG- |
| GCACAGTGCCACCATCAACTTGAactagtCCAG-3′ | |
| pGL3CM5 | 5′-GCACAGACTCTGTACTTTCCTGACCT- |
| TactagtGTGCCACCATCAACTTGACTGACACCAG-3′ | |
| pGL3CM3 | 5′-GCACAGACTCTGTACTTTCactagtTTG- |
| GCACAGTGCCACCATCAACTTGACTGACACCAG-3′ | |
| pGL3CM2 | 5′-GCACAGACTCTGTACTTTCgaattCTTG- |
| GCACAGTGCCACCATCAACTTGACTGACACCAG-3′ | |
| pGL3CS5 | 5′-CTCTGTACTTTCCTGACCTTCTCTG- |
| TACTTTCCTGACCTTCTCTGTACTTTCCTGACCTT-3′ |