TABLE 1

Cell lines and drug treatments used for DNA microarray experiments 25,940 genes were analyzed using Incyte GEM 1—5 DNA microarrays.

Cell Type Genotypea Treatment(s)b
HT29 colon cancer p53 —, APC —, MMR + 0.5 μM 5-aza-CdR, day 5
HCT116 colon cancer p53 +, APC +,c MMR — 0.5 μM 5-aza-CdR, day 5
RKO colon cancer p53 +, APC +, MMR — 0.5 and 2.0 μM 5-aza-CdR, day 5
Human mammary epithelial cells p53 +, APC +, MMR + 1.0 μM 5-aza-CdR (2×), days 4 and 9
  • a p53, adenomatous polyposis coli, and mismatch repair genotype are indicated (+ indicates wild type)

  • b 5-aza-CdR—treated cells were compared to vehicle (phosphate-buffered saline)-treated cells

  • c Activating β-catenin mutation