TABLE 1

HA FITC antibody binding to HEK 293 cells expressing 100 ng of HA hRAMPs or HA DN hRAMP mutants with or without 100 ng of CTR2 Data are presented as mean ± S.E. (n = 3).

	Permeabilized Cells		Nonpermeabilized Cells
	hCTR2 (—)	hCTR2 (+)	hCTR2 (—)	hCTR2 (+)
		%
Control	1.88 ± 0.03	1.98 ± 0.17	1.47 ± 0.11	1.85 ± 0.04
HA hRAMP1	53.6 ± 0.75^*	55.1 ± 0.92#	3.30 ± 0.23^*	26.2 ± 1.79^#^†
HA hRAMP2	57.5 ± 0.85^*	58.6 ± 1.71#	9.31 ± 0.36^*	16.7 ± 1.12^#^†
HA hRAMP3	53.8 ± 1.01^*	54.5 ± 0.37#	22.0 ± 0.48^*	32.5 ± 0.38^#^†
HA DN hRAMP1	51.5 ± 1.34^*	57.9 ± 1.44#†	4.64 ± 0.73^*	24.2 ± 0.43^#^†
HA DN hRAMP2	60.9 ± 2.06^*	62.6 ± 0.71#	12.0 ± 0.37^*	17.4 ± 0.52^#^†
HA DN hRAMP3	56.5 ± 0.72^*	59.2 ± 0.41#†	23.2 ± 1.02^*	32.5 ± 2.97^#^†

↵* P < 0.001 versus empty vector
↵# P < 0.001 versus hCTR2 alone
↵† P < 0.05 versus corresponding CRLR (—)