Ki | Stimulation of Intracellular Ca2+ EC50 | |||
---|---|---|---|---|
Alexa488 CCK | Alexa568-Receptor (CCK) | Alexa488-CCK | Alexa568-Receptor (CCK) | |
nM | ||||
Wild-type | 3.3 ± 1.3 | N.D. | 0.6 ± 0.1 | N.D. |
C94 | 7.0 ± 1.0 | 4.6 ± 1.5 | 0.6 ± 0.1 | 0.7 ± 0.2 |
N102C | 1.7 ± 0.6 | 1.4 ± 0.4 | 0.9 ± 0.1 | 1.1 ± 0.4 |
A204C | 12.5 ± 3.1 | 5.2 ± 3.8 | 0.4 ± 0.2 | 0.8 ± 0.2 |
T341C | 10.3 ± 2.6 | 2.0 ± 0.3 | 0.7 ± 0.2 | 0.9 ± 0.2 |
N.D., not determined, because these lanes represent monoderivatized receptor constructs that were not possible for the wild-type CCK receptor. The wild-type CCK receptor binds CCK with a Ki = 1.0 ± 0.2 nM.