TABLE 1

Km and Vmax values of dNTPs for HBV DNA polymerases with a wild-type RNA template and a G1896A-mutated RNA template Procedures were as described under Materials and Methods. Values are presented as mean ± S.D. of at least three independent experiments.

Variable Substrate DP KmVmax Relative Efficiency (Vmax/Km)
Wa Mb W M W M
nM 10-6 pmol/min/μg
dATP WT 130 ± 50 240 ± 10 4.20 ± 0.14 3.67 ± 0.72 100 100
L526M 290 ± 40 330 ± 100 0.56 ± 0.09 2.35 ± 0.78 5.9 47
M550V 390 ± 180 650 ± 10 0.32 ± 0.17 0.69 ± 0.35 2.5 9.2
L526M/M550V 220 ± 10 360 ± 120 0.42 ± 0.12 0.88 ± 0.37 5.9 16
dCTP WT 100 ± 7 110 ± 50 5.34 ± 2.48 5.50 ± 2.1 100 100
L526M 220 ± 80 170 ± 20 1.34 ± 0.09 3.45 ± 0.92 11 40
M550V 430 ± 150 270 ± 40 0.39 ± 0.14 0.56 ± 0.14 1.7 4.2
L526M/M550V 170 ± 100 280 ± 140 0.71 ± 0.14 1.44 ± 0.76 7.9 10
dGTP WT 50 ± 10 230 ± 70 4.29 ± 0.95 3.12 ± 0.39 100 100
L526M 130 ± 60 330 ± 60 0.27 ± 0.1 1.31 ± 0.6 2.4 28
M550V 220 ± 60 550 ± 120 0.11 ± 0.01 0.50 ± 0.14 0.6 6.6
L526M/M550V 160 ± 50 380 ± 90 0.21 ± 0.09 0.56 ± 0.21 1.5 11
dTTP WT 130 ± 40 260 ± 40 7.90 ± 1.2 2.98 ± 0.72 100 100
L526M 350 ± 90 310 ± 100 0.65 ± 0.21 2.19 ± 0.5 3.1 61
M550V 230 ± 110 280 ± 120 0.13 ± 0.03 0.82 ± 0.18 0.9 2.5
L526M/M550V 190 ± 20 280 ± 60 0.58 ± 0.10 1.21 ± 0.39 5.1 3.7

  • ↵ a Wild-type RNA template.

  • ↵ b G1896A-mutated RNA template.