Ki | Ki/Km | Cell Culture | ||||
---|---|---|---|---|---|---|
Substrate | DP | Wa | Mb | W | M | IC50 |
μM | nM | |||||
L(-)SddCTP | WT | 0.2 ± 0.02 | 0.4 ± 0.2 | 2 (1)c | 4 (1) | 5 (1) |
L526M | 2.9 ± 0.2 | 1.3 ± 0.3 | 14.5 (7) | 7.6 (2) | 35 (7) | |
M550V | 15.2 ± 1.8 | 7 ± 1 | 38 (19) | 26 (8.5) | ||
L526M/M550V | 8.2 ± 0.9 | 6.7 ± 0.5 | 41 (20) | 24 (6) | 8000 (1600) | |
L(-)Fd4CTP | WT | 0.9 ± 0.2 | 0.6 ± 0.3 | 9 (1) | 6 (1) | 0.9 (1) |
L526M | 1.4 ± 0.5 | 1 ± 0.4 | 7 (0.7) | 5.8 (1) | 2.7 (3) | |
M550V | 42.7 ± 7 | 20 ± 2.5 | 106 (12) | 74 (12) | ||
L526M/M550V | 7.1 ± 3 | 5 ± 2.2 | 35.5 (4) | 18 (3) | 210 (233) | |
L(-)FMAUTP | WT | 4.2 ± 2 | 1.3 ± 0.2 | 32 (1) | 5 (1) | 20 (1) |
L526M | 14.1 ± 4 | 6.4 ± 0.5 | 40 (1.3) | 20 (4) | 80 (4) | |
M550V | 58.5 ± 13 | 22 ± 5 | 254 (7.9) | 78 (15.6) | ||
L526M/M550V | 66.6 ± 8 | 25 ± 3.5 | 350 (11) | 89 (17.8) | 50,000 (2500) |