TABLE 1

C-terminal sequences of native human UGT1A9, recombinant full-length enzyme with a fusion peptide (1A9XHC), and the new mutant, UGT1A9Sol

The hydrophobic segment that forms the single trans-membrane helix of the enzyme is indicated in bold (A and B), and the sequences of the C-terminal extensions of 1A9XHC (Kurkela et al., 2003) and UGT1A9Sol are italic.

Protein C-Terminal Sequence
UGT1A9 W480YQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH530
1A9XHC W480YQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTHSEVDKDLYDDDDKDHHHHHH550
UGT1A9Sol W480YQYHSLDVDKDLYDDDDKDHHHHHH505