Molecular Mass | |||||
---|---|---|---|---|---|
Enzyme | Expression Level | Sp. Content | Expected | Observed | Δ |
nmol/l | nmol/mg | Da | Da | ||
CYP2C9 | 500 | 18.1 | 55,627 | 55,630 | +3 |
R97F | ≤50 | 5.3 | 55,618 | —a | — |
R97H | ≤100 | 8.8 | 55,608 | 55,610 | +2 |
R105F | 500 | 14.2 | 55,618 | 55,624 | +6 |
R105H | 500 | 14.3 | 55,608 | 55,612 | +4 |
R108F | 500 | 17.4 | 55,618 | 55,619 | +1 |
R108H | 800 | 15.7 | 55,608 | 55,610 | +2 |
D293N | 250 | 8.6 | 55,626 | 55,629 | +3 |
D293V | 200 | 8.1 | 55,611 | 55,616 | +5 |
↵ a Analysis not performed due to insufficient amounts of the protein.