TABLE 4

Thermal stability of CYP2C9 mutants

Enzyme % P420 (at 0 min) Half-Life
min
wt2C9 0 16
R97F —a —a
R97H 30 <2b
R105F 0 17
R105H 0 13
R108F 0 24
R108H 9 177
D293N 0 2
D293V 0 3

  • ↵ a Analysis was not performed due to insufficient amounts of protein.

  • ↵ b No remaining P450 peak was observed after a 5-min preincubation at 48°C.