Percentage maximum responses for the rate of SPAP secretion between 5 and 6 h after the addition of agonists
The agonists were incubated with the cells for 10 min to 5 h, the agonists were washed out, and 100 nM ICI 118551 was incubated for the remaining time up to a total of 5 h of incubation. SPAP secretion between 5 and 6 h was then measured. Values represent means ± S.E.M. of n determinations.
| % Isoprenaline | % of Maximal Agonist Response | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Agonist | 5 h | n | 4 h | n | 3 h | n | 2 h | n | 1 h | n | 30 min | n | 10 min | n |
| Isoprenaline | 100 | 10 | 92.3 ± 9.4 | 3 | 93.3 ± 11.6 | 3 | 73.4 ± 6.1 | 7 | 44.5 ± 1.5 | 10 | 25.6 ± 2.0 | 11 | N.R. | 10 |
| Salmeterol | 111.5 ± 3.6 | 4 | 101.8 ± 10.8 | 4 | 89.1 ± 8.8 | 4 | 85.6 ± 7.8 | 4 | 64.3 ± 0.2 | 4 | 54.4 ± 3.8 | 4 | 52.1 ± 8.0 | 4 |
| Alprenolol | 53.2 ± 6.6 | 4 | 80.8 ± 14.3 | 4 | 82.8 ± 6.7 | 4 | 61.4 ± 5.1 | 4 | 41.0 ± 1.7 | 4 | 25.7 ± 3.2 | 4 | 13.1 ± 2.7 | 3 |
| CGP 12177 | 52.8 ± 3.5 | 4 | 95.0 ± 11.8 | 4 | 100.8 ± 9.5 | 4 | 91.9 ± 3.8 | 4 | 75.4 ± 5.1 | 4 | 48.6 ± 9.5 | 4 | 36.4 ± 5.3 | 4 |
% isoprenaline, maximum 5-h response of each ligand expressed as a percentage of the maximum 5-h response to 10 μM isoprenaline as determined in each experiment; % of maximal agonist response, response of that ligand expressed as a percentage of the maximum 5-h response for that same ligand; N.R., no response seen.