Drugs that do not alter the M3 mAChR-induced inhibition of proliferation
Uptake of [3H]thymidine by m3Zeo-2 and cells expressing wild-type or mutant HA-Rac1 proteins was measured after the cells were cultured for 24 h in the absence or presence of the indicated concentrations of the listed drugs and in the absence or presence of 10 μM CCh. The results of three independent experiments using each drug were used to compile data for this table. Enzymes listed in parentheses are considered secondary targets of the indicated drug (Davies et al., 2000).
| Drug | Target Enzyme | Concentrations Tested |
|---|---|---|
| μM | ||
| AACOCF3 | Phospholipase A2 | 6.25—200 |
| D609 | Phosphatidylcholine-specific phospholipase C (mitogen-activated protein kinase) | 2.5—80 |
| KN-93 | CaM kinase II | 0.625—20 |
| W-7 | CaM kinase II MLC kinase | 3.125—100 |
| U0126 | MEK1 and MEK2 (p38, protein kinase B) | 1.56—50 |
| LY 294002 | Phosphatidylinositol 3-kinase (casein kinase 2) | 1.56—50 |
| Wortmannin | Phosphatidylinositol 3-kinase (myosin light chain kinase, PI4 kinase) | 0.313—50 |