Regioselective composition of EETs and HETEs produced by recombinant CYP2C enzymes
The regiochemical distribution of EETs and HETEs formed by incubation of recombinant mouse CYP2C enzymes with [1-14C]AA was quantified by HPLC and liquid scintillation as described under Materials and Methods. Values shown are average of at least three different experiments.
| Distribution | |||
|---|---|---|---|
| Regioisomer | CYP2C50 | CYP2C54 | CYP2C55 |
| % | |||
| HETEs | |||
| 5-HETE | 3.5 | 11.5 | N.D. |
| 8-HETE | 12.7 | 2.1 | N.D. |
| 9-HETE | N.D. | N.D. | 1.6 |
| 11-HETE | 17.3 | 6.7 | 2.2 |
| 12-HETE | 23.6 | 55.9 | 3.6 |
| 15-HETE | 12.9 | 6.4 | 4.2 |
| 16-HETE | 12.6 | N.D. | N.D. |
| 17-HETE | N.D. | N.D. | N.D. |
| 18-HETE | 5.0 | 6.6 | 7.0 |
| 19-HETE | 12.3 | N.D. | 78.6 |
| 20-HETE | N.D. | 11.0 | 3.0 |
| EETs | |||
| 5,6-EET | 12 | 20 | 7 |
| 8,9-EET | 23 | 25 | 18 |
| 11,12-EET | 36 | 30 | 40 |
| 14,15-EET | 29 | 25 | 36 |
N.D., not detectable at a detection limit of 1% (∼100 cpm).