Kinetic analysis of the reaction of O<bardot;2> with NQO1
O<bardot;2> was generated using xanthine/XO as described under Materials and Methods. Results are the mean ± S.D. of three separate determinations.
| Parameter | Value |
|---|---|
| Km (μM) | 2.87 ± 0.90 |
| Vmax (pmol/min) | 4021 ± 361 |
| Kcat (min−1) | 3.10 ± 0.28 |
| Kcat/Km (M/s) | 3.60 × 104 |