Comparison of the effects of nanomolar [suramin] and CaM on the open and closed lifetime distributions of single cardiac RyR channels in the presence of 100 μM cytosolic Ca2+
Values are mean ± S.D. for n = 3.
| τ1 | Area | τ2 | Area | τ3 | Area | |
|---|---|---|---|---|---|---|
| ms | % | ms | % | ms | % | |
| Open lifetime parameters | ||||||
| 100 μM Ca2+ | 2.3 ± 0.7 | 93.0 ± 3.8 | 17.1 ± 2.0 | 6.7 ± 3.8 | ||
| 500 nM suramin | 2.5 ± 0.5 | 86.7 ± 2.0 | 28.6 ± 12.8 | 13.3 ± 2.0 | ||
| 50 nM CaM | 1.8 ± 0.9 | 93.0 ± 3.6 | 22.5 ± 17.9 | 7.0 ± 3.6 | ||
| Closed lifetime parameters | ||||||
| 100 μM Ca2+ | 3.9 ± 0.6 | 65.5 ± 14.4 | 11.5 ± 2.0 | 28.7 ± 15.1 | 30 ± 9.76 | 5.7 ± 2.5 |
| 500 nM suramin | 5.0 ± 1.7 | 48.6 ± 8.5 | 32.6 ± 9.9 | 39.3 ± 3.5 | 223 ± 156 | 12.0 ± 5.0 |
| 50 nM CaM | 5.8 ± 1.3 | 33.0 ± 20.8 | 45.5 ± 12.6 | 48.3 ± 7.5 | 232 ± 103 | 18.7 ± 13.3 |