Effects of flavonoids on the accumulation of mitoxantrone in both parent and mitoxantrone-selected MCF-7 and NCI-H460 cells
The cellular accumulation of mitoxantrone in MCF-7 and NCI-H460 cells (both parent and drug-selected) in the absence or presence of flavonoids (50 μM) was performed as described under Materials and Methods. FTC (10 μM) was used as a positive control. The accumulation of mitoxantrone was expressed as percent of control (in the presence of the vehicle, 0.1% DMSO). The data were expressed as mean ± S.D., n = 6 or 8.
| MCF-7/Sensitive | MCF-7 MX100 | NCI-H460 | NCI-H460 MX20 | |
|---|---|---|---|---|
| Control | 100 ± 13.4 | 100 ± 14.8 | 100 ± 4.42 | 100 ± 11.0 |
| Apigenin | 88.6 ± 9.85 | 321 ± 36.1*** | 89.0 ± 10.8 | 355 ± 36.2*** |
| Biochanin A | 114 ± 12.4 | 440 ± 99.5*** | 129 ± 14.2*** | 463 ± 59.7*** |
| Chrysin | 98.5 ± 8.03 | 389 ± 94.1*** | 97.4 ± 15.0 | 367 ± 35.5*** |
| Daidzein | 106 ± 10.9 | 215 ± 13.1*** | 93.8 ± 18.3 | 297 ± 23.1*** |
| EGC | 101 ± 10.6 | 155 ± 19.1 | 73.5 ± 11.9* | 95.6 ± 15.0 |
| EGCG | 52.9 ± 8.78*** | 132 ± 15.6 | 25.7 ± 2.92*** | 34.4 ± 8.00 |
| Fisetin | 105 ± 16.1 | 223 ± 33.2*** | 78.4 ± 10.0 | 226 ± 37.0*** |
| Genistein | 104 ± 12.9 | 412 ± 47.0*** | 105 ± 10.1 | 331 ± 54.0*** |
| Hesperetin | 112 ± 15.9 | 362 ± 55.2*** | 101 ± 26.8 | 305 ± 55.8*** |
| Kaempferol | 85.2 ± 11.3 | 378 ± 17.3*** | 80.5 ± 10.4 | 301 ± 42.5*** |
| Luteolin | 69.5 ± 10.2*** | 155 ± 19.5 | 63.7 ± 8.48*** | 213 ± 23.6*** |
| Morin | 60.2 ± 9.25*** | 186 ± 33.8 | 45.8 ± 6.92*** | 153 ± 30.3 |
| Myricetin | 86.5 ± 11.8 | 102 ± 17.5 | 52.0 ± 5.40*** | 75.2 ± 16.3 |
| Naringenin | 109 ± 11.8 | 345 ± 46.4*** | 102 ± 17.05 | 338 ± 28.0*** |
| Naringin | 107 ± 6.09 | 149 ± 62.1 | 98.7 ± 6.66 | 102 ± 23.2 |
| Phloretin | 94.8 ± 6.84 | 216 ± 44.1*** | 96.9 ± 12.3 | 304 ± 33.9*** |
| Phloridzin | 97.7 ± 11.7 | 114 ± 14.6 | 95.2 ± 12.5 | 78.0 ± 14.3 |
| Quercetin | 80.3 ± 8.64 | 206 ± 18.3* | 71.6 ± 5.52*** | 233 ± 18.4*** |
| Silibin | 105 ± 18.5 | 209 ± 19.7* | 128 ± 13.6*** | 331 ± 41.4*** |
| Silymarin | 103 ± 11.9 | 302 ± 60.9*** | 104 ± 18.4 | 344 ± 70.7*** |
| FTC | 116 ± 9.30 | 439 ± 89.8*** | 124 ± 19.8* | 404 ± 40.4*** |