P450 2B11: steady-state kinetics analysis of CPA and IFA metabolism
Data are based on n = 2 independent experiments.
| 4-Hydroxylation | N-Dechloroethylation | ||||||
|---|---|---|---|---|---|---|---|
| Substrate | Km | Vmax | Vmax/Km | Km | Vmax | Vmax/Km | % N-deCla |
| mM | mol/min/mol | mol/min/mol | mM | mol/min/mol | mol/min/mol | ||
| P450 | P450/mM | P450 | P450/mM | ||||
| CPA | 0.16 ± 0.01 | 28.2 ± 0.2 | 174.7 ± 8.4 | N.D. | 0 | N.D. | 0 |
| IFA | 0.08 ± 0.02 | 5.3 ± 0.5 | 66.8 ± 19.1 | 0.09 ± 0.01 | 5.0 ± 1.0 | 56.2 ± 5.8 | 48.2 ± 2.8 |
N.D., not determined; catalytic activity at or below limit of detection.
↵ a N-dechloroethylation as a percentage of total metabolism at Vmax.