EMSA and mutagenesis oligonucleotides Mutated nucleotides are underlined.
| No. | Oligonucleotides | Oligonucleotide Sequence (5′→3′) |
|---|---|---|
| EMSA Oligos | ||
| 48 | Cyp2c29-1370F | CTAGTTGGGTCACTGAAGTTCTGA |
| 53 | Cyp2c29-1370R | CTAGTCAGAACTTCAGTGACCCAA |
| 50 | Cyp2c29-1337F | CTAGAGTAAACTTTTTTGACTTCT |
| 51 | Cyp2c29-1337R | CTAGAGAAGTCAAAAAAGTTTACT |
| CYP2B6-NR1F | CTAGACTGTACTTTCCTGACCCTG | |
| CYP2B6-NR1R | CTAGCAGGGTCAGGAAAGTACAGT | |
| Mutagenesis Primers | ||
| 58 | MUT_NR1_2c29F | GATAACTGTGACATTGGGGGGCTGAAGTTCTGAACAT |
| 60 | MUT_NR1_2c29R | ATGTTCAGAACTTCAGCCCCCCAATGTCACAGTTATC |
| 59 | MUT_NR2_2c29F | CATTACAAAACAAGTAATAATTTTTGACTTCTAAGTTAA |
| 61 | MUT_NR2_2c29R | TTAACTTAGAAGTCAAAAATTATTACTTGTTTTGTAATG |
| 64 | c/EBPmut_2c29F | TCACTGAAGTTCTGAACACCATAAGACAAGTAAACTTTTTTGACT |
| 65 | c/EBPmut_2c29R | AGTCAAAAAAGTTTACTTGTCTTATGGTGTTCAGAACTTCAGTGA |