Induced mutation frequencies for cisplatin and nitrogen mustard treatments
Exposures that yielded between 1 and 5% survival were used to calculate mutation frequencies. The only exception was nitrogen mustard, where wild-type and TLS-compromised cells yielded 10 to 20% survival. Frequencies are expressed as recombinants or mutants per surviving cell. Numbers in parentheses indicate the average fold increases over control (untreated).
| Mutation Frequency (× 10−6) | |||
|---|---|---|---|
| Relevant Genotype | Cisplatin | Nitrogen Mustard | Untreated Cells |
| WT | 19.5 ± 3.7 (11) | 13.3 ± 1.2 (6.5) | 1.88 ± 0.18 |
| NER-compromised | 7.62 ± 3.8 (1.3) | 36.9 ± 12 (6.4) | 5.86 ± 0.32 |
| REC-compromised | 31.0 ± 0.90 (1.4) | 29.2 ± 0.80 (1.4) | 22.1 ± 0.97 |
| TLS-compromised | 0.955 ± 0.42 (0.87) | 0.994 ± 0.36 (0.83) | 1.20 ± 0.30 |
| NER/REC-compromised | 61.6 ± 17 (0.68) | 133 ± 14 (1.5) | 95.9 ± 16 |
WT, wild type.