Curve-fit values for wild-type and T6′F mutant α7 responses to ACh
Data represent curve values generated by the Hill equation (see Materials and Methods) for macroscopic responses from oocytes expressing either indicated receptor.
| Normalized Imax | Hill Coefficient | EC50 | |
|---|---|---|---|
| Rat α7 wild-type | |||
| ACh peaka | |||
| ACh net charge | 1.05 ± 0.02 | 1.5 ± 0.1 | 16.5 ± 1.0 μM |
| Rat α7 T6′F mutant | |||
| ACh peak | 0.89 ± 0.04 | 1.5 ± 0.4 | 1.7 ± 0.4 μM |
| ACh net charge | 0.96 ± 0.04 | 2.4 ± 1.1 | 1.1 ± 0.2 μM |
↵ a The use of peak responses has been shown to provide inaccurate estimates of these parameters (Papke and Papke, 2002) and curve-fitting was thus restricted to net charge analysis for wild-type α7.