TABLE 1

Amino acid sequence of the peptides used to produce nAChR subunit-specific polyclonal antibodies

Amino acid sequence of the peptides used to produce the subunit-specific antibodies. Capital letters indicate the amino acids present in the subunit sequence, whereas the lowercase letters indicate the extra-sequence amino acids introduced to enable specific coupling to carrier protein.

Subunit Peptide Sequence Localization Species
α2 CHPLRLKLSPSYHWLESNVDAEEREV CYT Human
α3 TRPTSNEGNAQKPRPLYGAELSNLNC CYT Human
α3 CQPLMARDDT COOH Rat
α4 PTSSPTSLKARPSQLPVSDQASPC CYT Rat
α4 cgPPFLAGMI COOH Rat
α5 DRYFTQREEAESGAGPKSRNTLEAALDC CYT Rat
α5 cgPVHIGNANK COOH Rat
α6 (1) GVKDPKTHTKRPAKVKFTHRKEPKLLKEC CYT Rat
α6 (2) CHKSSEIAPGKRLSQQPAQWTENSEHPPDV CYT Rat
β2 RQREREGAGALFFREAPGADSCT CYT Human
β2 cgLHSDHSAPSSK COOH Rat
β3 CGKESDTAVRGKVSGKRKQTPASD CYT Rat
β3 cgPALKMWIHRFH COOH Rat
β4 VSSHTAGLPRDARLRSSGRFREDLQEALEGc CYT Rat
β4 cGLPPLFQIHAPSKDS COOH Rat