TABLE 3

Pregnanolone-dependent modulation of Ile307 mutants in the presence their respective EC9 GABA

All data are presented as mean ± S.E.M.

Subunit 0.1 μM 0.5 μM 1 μM 5 μM 10 μM 20 μM n
ρ1* −2 ± 2 −18 ± 5 −29 ± 5 −45 ± 6 −46 ± 5 −34 ± 5 4
I307V −1 ± 3 −31 ± 7 −37 ± 5 −45 ± 2 −32 ± 1 −16 ± 1 3
I307C −11 ± 4 −47 ± 6 −67 ± 4 −64 ± 4 −41 ± 2 16 ± 3 4
I307T −19 ± 7 −54 ± 5 −63 ± 5 −63 ± 3 −33 ± 6 70 ± 6 5
I307S −6 ± 6 −28 ± 3 −56 ± 4 −43 ± 2 −9 ± 6 173 ± 20 3
I307A −27 ± 2 −54 ± 4 −53 ± 3 −45 ± 4 14 ± 12 172 ± 36 4
I307G −12 ± 9 −54 ± 4 −63 ± 6 −49 ± 6 63 ± 20 206 ± 41 4
I307E −7 ± 2 −18 ± 2 −25 ± 3 1 ± 3 66 ± 14 151 ± 18 6
I307M −7 ± 2 −4 ± 6 −2 ± 4 32 ± 8 95 ± 29 182 ± 42 3
I307N −10 ± 5 −28 ± 12 −26 ± 6 41 ± 9 130 ± 30 337 ± 103 4
I307W −5 ± 2 −12 ± 1 −9 ± 5 66 ± 8 299 ± 21 889 ± 73 3
I307R −11 ± 5 −16 ± 3 14 ± 15 152 ± 38 364 ± 72 508 ± 33 3
I307H −2 ± 4 2 ± 5 5 ± 3 62 ± 5 139 ± 14 329 ± 19 3
I307Q 3 ± 1 13 ± 5 23 ± 8 125 ± 22 249 ± 31 531 ± 66 4
R287A −5 ± 1 −41 ± 2 −54 ± 3 −63 ± 2 −53 ± 5 −8 ± 15 3
P294A −8 ± 1 −15 ± 1 −33 ± 3 −40 ± 3 −37 ± 3 −30 ± 5 5