TABLE 2

Ligand binding characteristics of C-terminal α1A-adrenoceptor splice variants

C-terminal splice variants of the human α1A-adrenoceptor were expressed either individually or in combination in HEK293 cells. Membranes were prepared, and saturation [3H]prazosin binding studies were performed. Data represent means ± S.E.M. from three independent experiments.

Receptor [3H]prazosin Kd
nM
α1A-1-AR 0.28 ± 0.04
α1A-2a-AR 0.41 ± 0.12
α1A-3a-AR 0.32 ± 0.07
α1A-1-AR + α1A-2a-AR 0.32 ± 0.03
α1A-1-AR + α1A-3a-AR 0.28 ± 0.05
α1A-2a-AR + α1A-3a-AR 0.35 ± 0.04