Surface plasmon resonance analysis of E522K DNA binding The average values of association (ka) and dissociation (kd) rate constants for binding of βE522K to three different DNA substrates. The values of the rate constants shown here are the mean value from 12 experiments; the S.D. from the mean value is shown in parenthesis. The equilibrium constants Ka and Kd were estimated using the experimental values of the association (ka) and dissociation (kd) rate constants. The average Kd for βwt is shown. This βwt* data was previously reported in Leontiou et al. (2003).
| DNA substrate | ka | Kd | Ka | Kd | βwt × Kd |
|---|---|---|---|---|---|
| M-1s-1 × 106 | s-1 × 10-3 | M-1 × 108 | M × 10-9 | ||
| 40 bp | 1.40 (± 0.61) | 2.30 (± 0.46) | 6.08 | 1.64 | 1.73 |
| 4wj | 1.26 (± 0.25) | 1.79 (± 0.05) | 7.04 | 1.42 | 2.85 |
| Bent | 1.38 (± 0.57) | 1.47 (± 0.32) | 9.38 | 1.06 | 1.86 |