TABLE 1

Time course analysis of cell cycle perturbations and apoptosis induced by equitoxic concentrations of TMZ in TK6 and MT1 cell lines The cells were incubated in CM or in CM containing 12.5 μM TMZ (TK6 cells) or 1.2 mM TMZ (MT1 cells) for 48 h. At the indicated time, the cells were processed for analysis of cell cycle perturbation and apoptosis induction. The percentage of cells in each phase of the cell cycle or in apoptosis was evaluated by flow cytometric analysis of DNA content. Data represent the means of four independent experiments, with S.E. not exceeding 10 to 15% of the mean.

Drug	Hours	TK6				MT1
Drug	Hours	Apoptosis	G₁/G₀	S	G₂/M	Apoptosis	G₁/G₀	S	G₂/M
					%
None	6	10	31	54	15	9	35	53	12
TMZ		12	30	56	14	9	31	63	6
None	12	11	30	60	10	8	36	54	10
TMZ		13	27	58	15	10	20	71	9
None	18	12	28	61	11	8	31	59	10
TMZ		12	24	61	15	12	19	72	9
None	24	11	29	59	12	6	33	56	11
TMZ		14	18	65	17	17	18	66	16
None	30	11	28	59	13	6	32	57	11
TMZ		20	12	72	16	20	22	56	22
None	36	8	28	62	10	4	35	54	11
TMZ		26	10	74	16	24	31	41	28
None	42	8	26	63	11	5	30	59	11
TMZ		34	16	66	18	28	38	42	20
None	48	7	26	62	12	4	32	57	11
TMZ		40	16	64	20	31	38	45	17