TABLE 1

Effect of P-gp modulators on the sensitivity of NH125 Cell lines grown in 96-well tissue culture plates were treated with varying concentrations of NH125 in the presence or absence of 5 μM modulator; viability was measured using an MTT assay as described under Materials and Methods. Each value represents the mean ± S.E. of the mean of at least two experiments.

Cell Line Drug(s) IC50
μM
A2780 NH125 1.60 ± 0.9
NH125+Verapamil 1.64 ± 1.1
NH125+trans-flupenxithol 1.59 ± 0.9
A2780-DX NH125 8.10 ± 1.6
NH125+Verapamil 2.78 ± 1.3
NH125+trans-flupenxithol 2.80 ± 0.9
MCF-7 NH125 2.70 ± 1.1
NH125+Verapamil 2.78 ± 0.9
NH125+trans-flupenxithol 2.50 ± 1.2
MCF-7/ADR NH125 4.84 ± 0.5
NH125+Verapamil 2.42 ± 0.3
NH125+trans-flupenxithol 2.34 ± 0.2