Induction of radiosensitization in the DC3F and DC3F/C10 cells by camptothecin (CPT) and the INDO derivatives F1 and F7 Radiation dose at 10% Survival is determined graphically from clonogenic survival assays conducted with cells pre-incubated with drug for 1 h followed by treatment with 0-, 2.5-, 5-, or 7.5-Gy doses of radiation. SER indicates the radiation dose at 10% survival without drug pretreatment divided by radiation dose at 10% survival with 30-min drug pretreatment.
| Drugs | DC3F Cells | DC3F/C10 Cells | ||
|---|---|---|---|---|
| Radiation Dose at 10% Survivala | SER | Radiation Dose at 10% Survival | SER | |
| Gy | Gy | |||
| No Drug Control Camptothecin | 6.0 | 1.0 | 5.2 | 1.0 |
| 0.1 μM | 4.3 | 1.4 | N.D. | N.D. |
| 1.0 μM | 3.9 | 1.5 | 5.2 | 1.0 |
| 10.0 μM | N.D. | N.D. | 4.6 | 1.1 |
| F1 | ||||
| 1.0 μg/ml | 4.9 | 1.2 | N.D. | N.D. |
| 5.0 μg/ml | 4.6 | 1.3 | 4.8 | 1.1 |
| 25.0 μg/ml | N.D. | N.D. | 4.5 | 1.2 |
| F7 | ||||
| 2.5 μg/ml | 4.6 | 1.3 | N.D. | N.D. |
| 5.0 μg/ml | 3.9 | 1.5 | 5.2 | 1.0 |
| 10.0 μg/ml | 3.6 | 1.7 | 4.9 | 1.1 |
| 20.0 μg/ml | N.D. | N.D. | 4.1 | 1.3 |
N.D., not determined.