Oligonucleotide primers used in the reverse transcription-PCR
| Gene (Accession Number) | Forward Primer | Reverse Primer | Product Length | Cycler Program |
|---|---|---|---|---|
| bp | ||||
| Calsequestrin (u33287) | tca aag acc cac cct acg tc | cca gtc ttc cag ctc ctc ag | 352 | 57°C, 60 s/30 cycles |
| Calreticulin (NM 022399.1) | atgaccccacagattccaag | gcataggcctcatcattggt | 339 | 55°C, 60 s/33 cycles |
| Calmodulin (AF 178845.1) | gaagcaggccagtcaaagac | cgaatttggaagccaacact | 348 | 55°C, 60 s/33 cycles |
| SERCA 2a (van den Bosch et al., 1994) | tcc atc tgc ctg tcc at | gcggttactccagtattg | 196 | 55°C, 60 s/35 cycles |
| SERCA 2b (van den Bosch et al., 1994) | tcc atc tgc ctg tcc at | aga cca gaa cat atc act | 324 | 55°C, 60 s/35 cycles |
| PMCA 1 (j03753) | tgc ctt gtt ggg att tct ct | cac tct ggt tct ggc tct cc | 351 | 55°C, 60 s/35 cycles |
| PMCA 2 (j03754) | att gat gga gct ggg atc ag | act tca ccg tgg aca cct tc | 350 | 57°C, 60 s/35 cycles |
| PMCA 4 (u15408) | agc agt tgc gtc agt cag aa | gct ttg tag agg gct gtt gg | 351 | 57°C, 60 s/38 cycles |
| Na-K-ATPase (NM012504) | tgt gat tct ggc tga gaa cg | agg aca gga aag cag caa ga | 299 | 57°C, 60 s/35 cycles |
| NCX-1 (x68191) | tgt ctg cga ttg ctt gtc tc | tca ctc atc tcc acc aga cg | 364 | 55°C, 60 s/35 cycles |
| Kir 3.4 (L35771) | gga tgg cag aca gga aag ag | acc acc tgt tgg agc tgt tc | 351 | 56°C, 60 s/35 cycles |
| Kir 6.1 (D42145) | tga gtc tag gac gcg ttg tg | cac cag cca cca cat gat ag | 351 | 55°C, 60 s/35 cycles |
| Nav 1.5 (M27902) | cag ctg aca tga cca aca cc | ttg agc agc atc tcc aac ac | 349 | 55°C, 60 s/35 cycles |
| RYR 2 (af130880) | cca aca tgc cag acc cta ct | ttt ctc cat cct ctc cct ca | 348 | 57°C, 60 s/35 cycles |
| RYR 3 (af130881) | cgt ctc tgg tgt cat ggc ta | cac act tca tat ccg gct ca | 345 | 57°C, 60 s/38 cycles |
| Phospholamban (x71068) | gct gag ctc cca gac ttc ac | gcg aca gct tgt cac aga ag | 339 | 57°C, 60 s/30 cycles |
| Troponin T (NM012676.1) | gggtacatccagaaggctca | gtgcctggcaagacctagag | 374 | 55°C, 60 s/33 cycles |
| Troponin I (NM017144.1) | atctccgcctccagaaaact | cagtagtgcctgcatcatgg | 352 | 55°C, 60 s/33 cycles |
| α-Cardiac Actin (X80130) | actcctatgtaggtgacgaggc | gacgttatgagtcacaccgtcg | 337 | 57°C, 60 s/33 cycles |
| α-Skeletal Actin (V01218) | atctcacgttcagctgtggtca | accaccggcatcgtgttggat | 182 | 55°C, 60 s/33 cycles |
| α-MHC (X15938) | ggaagagcgagcggcgcatcaagg | ctgctggacaggttattcctca | 304 | 65°C, 60 s/35 cycles |
| β-MHC (X15939) | gccaacaccaacctgtccaagttc | ttcaaaggctccaggtctcagggc | 202 | 55°C, 60 s/35 cycles |
| GAPDH (J02624) | ggccaaggtcatccatga | tcagtgagcccaggatg | 353 | 55°C, 60 s/32 cycles |