TABLE 1

Internal solutions used in this study The concentration of each substance is indicated in millimolar concentration. The pH of all solutions was adjusted to 7.3.

Internal Solution KCl NaCl MgCl2 EGTA EDTA HEPES
A 140 0 2a 5 0 5
B 140 0 0 0 5 5
C 140—xb x b 0 0 5 5

  • ↵ a When 1 mM ATP was added to the internal solution A, 1 mM MgCl2 was further added to maintain the free Mg2+ concentration

  • ↵ b Value of x was 30, 60, 90, or 120