Binding affinities of [3H]SQ29,548 in HEK293 cells transiently expressing the wild-type or the mutant human TPα receptors Binding affinities and capacities were obtained by simultaneous analysis with the LIGAND program of several independent [3H]SQ29,548 mixed-type experiments.
| Receptor | Kd | Bmax | No. of Experiments |
|---|---|---|---|
| nM ± %CV | pmol/mg protein ± %CV | ||
| Wild type | 6.39 ± 12 | 0.54 ± 11 | 9 |
| R130K | 5.32 ± 10 | 0.67 ± 8 | 4 |
| R130V | 7.89 ± 24 | 0.69 ± 18 | 3 |
| E129V | 5.38 ± 9 | 0.46 ± 7 | 5 |
| E129D | 6.32 ± 16 | 0.45 ± 12 | 3 |
| Wild type-GFP | 8.96 ± 33 | 0.82 ± 23 | 2 |
| R130V-GFP | 10.38 ± 11 | 0.66 ± 8 | 2 |