Pentobarbital (100 μ M) modulated the open states of GABAA receptors For α1β3γ2L receptors, the average number of openings were 3758 (GABA), 3899 (GABA + pentobarbital), and 3645 (pentobarbital); and for α1β3δ receptors, 5309 (GABA), 4320 (GABA + pentobarbital), and 3499 (pentobarbital).
| α1β3γ2L | α1β3δ | |||||
|---|---|---|---|---|---|---|
| GABA (1 mM) | + PB (100 μM) | PB (100 μM) | GABA (1 mM) | + PB (100 μM) | PB (100 μM) | |
| n | 5 | 5 | 4 | 6 | 7 | 5 |
| Mean | 1.45 ± 0.26 | 4.86 ± 1.06a,c | 1.41 ± 0.19 | 0.53 ± 0.04 | 1.08 ± 0.17a,c | 0.48 ± 0.07 |
| τ1 (ms) | 0.31 ± 0.02 | 0.32 ± 0.07 | 0.24 ± 0.01f | 0.33 ± 0.02 | 0.35 ± 0.03e | 0.18 ± 0.02g |
| A1 (%) | 49.2 ± 3.33 | 33.2 ± 5.34a | 46.0 ± 6.39 | 65.0 ± 2.87 | 54.4 ± 2.81a | 61.1 ± 3.26 |
| τ2 (ms) | 1.55 ± 0.39 | 2.28 ± 0.58 | 1.11 ± 0.13 | 0.90 ± 0.10 | 1.27 ± 0.10a,d | 0.75 ± 0.10 |
| A2 (%) | 32.7 ± 3.01 | 25.4 ± 3.33 | 32.9 ± 2.92 | 35.1 ± 2.88 | 35.3 ± 1.97 | 35.4 ± 2.87 |
| τ3 (ms) | 4.63 ± 0.81 | 9.37 ± 1.11b,c | 4.66 ± 0.67 | 4.03 ± 0.59 | 2.95 ± 0.62 | |
| A3 (%) | 18.2 ± 3.04 | 41.4 ± 7.21a | 21.2 ± 4.33 | 10.3 ± 2.15c | 3.50 ± 0.57 | |
PB, pentobarbital.
↵ a Significantly different from corresponding GABA control at p < 0.05
↵ b Significantly different from corresponding GABA control at p < 0.01
↵ c Significantly different from corresponding PB at p < 0.05
↵ d Significantly different from corresponding PB at p < 0.01
↵ e Significantly different from corresponding PB at p < 0.001
↵ f Significantly different from corresponding GABA at p < 0.05
↵ g Significantly different from corresponding GABA at p < 0.001